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公司介绍
stemRD及其合作伙伴已经开发出一种高效的方法,可以在人细胞系中表达生物活性重组蛋白。由于这些细胞在无血清,无蛋白质和化学成分明确的培养基中生长,因此通过此过程制得的产品不含异种,并且具有人类特异性的转录后修饰,例如糖基化。使用该系统,我们已经能够生产出许多对干细胞的生长和分化至关重要的生物活性蛋白。
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  • URL:http://stemrd.com/

Stemrd/WNT-5a,小鼠/5µg/W5A-M-005

  • 产品编号: W5A-M-005
  • 美  元  价: 2450.00
  • 市  场  价: ¥4900.00
  • 品       牌: Stemrd
  • 产       地: 美国(厂家直采)
  • 公       司:
  • 产品分类: 抗小鼠>
  • 公司分类: Anti_mouse
Stemrd/WNT-5a,小鼠/5µg/W5A-M-005
  • Stemrd/WNT-5a,小鼠/5µg/W5A-M-005
商品介绍
SourceM.W.~45 kDa CAS No.
Structural Info
FormulationLyophilized in sterile filtered solution of PBS with 2% CHAPS
ReconstitutionBefore reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containingcarrier proteins, such as albumin or serum.
StabilityThe lyophilized protein isstable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least1 month at 4 °C, but should be stored in aliquots at -80 °C forlonger term. Avoidrepeated freeze and thaw.
PurityGreater than 85% as determined by SDS-PAGE and HPLC analysis
Biological ActivityThe activity was determined by using a TCF reporter gene assay in 293 cells co-transfected with Frizzled-4 and LRP-5.WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay.
Country of OriginUSA

WNT-5a belongs to the class of WNT proteins thatactivate the “non-canonical” pathway. Thepredicted size of mouse WNT-5a is a monomeric protein containing 356 amino acidresidues. Due to glycosylation, itmigrates at an apparent molecular weight of ~45 kDa by SDS-PAGE analysis undernon-reducing conditions. StemRD’sproduct is expressed from a mouse cell line, and purified with a proprietaryprocess that is distinct from the published method.

Product Sheet
Ishida-Takagishi M, Enomoto A, etal., The Dishevelled-associating protein Daple controls the non-canonicalWnt/Rac pathway and cell motility. NatureCommunication. 2012May 29;3:859 http://www.ncbi.nlm.nih.gov/pubmed/22643886Nakamuta S, Funahashi Y, Namba T etal., Local application of neurotrophins specifies axons throughinositol 1,4,5-trisphosphate, calcium, and Ca2+/calmodulin-dependentprotein kinases. ScienceSignal. 2011 Nov 15;4(199):ra76.http://www.ncbi.nlm.nih.gov/pubmed/22087032
品牌介绍

stemRD及其合作伙伴已经开发出一种高效的方法,可以在人细胞系中表达生物活性重组蛋白。由于这些细胞在无血清,无蛋白质和化学成分明确的培养基中生长,因此通过此过程制得的产品不含异种,并且具有人类特异性的转录后修饰,例如糖基化。使用该系统,我们已经能够生产出许多对干细胞的生长和分化至关重要的生物活性蛋白。

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