4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:原厂直采,平行进口,授权代理(蚂蚁淘为您服务)
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Cell_transfection > Stemrd/WNT-5a,人/5µg/W5A-H-005
商品详细Stemrd/WNT-5a,人/5µg/W5A-H-005
Stemrd/WNT-5a,人/5µg/W5A-H-005
Stemrd/WNT-5a,人/5µg/W5A-H-005
商品编号: W5A-H-005
品牌: Stemrd
市场价: ¥6900.00
美元价: 3450.00
产地: 美国(厂家直采)
公司:
产品分类: 细胞转染
公司分类: Cell_transfection
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
SourceM.W.~45 kDa CAS No.
Structural Info
FormulationLyophilized in sterile filtered solution of PBS with 1% CHAPS
ReconstitutionBefore reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containingcarrier proteins, such as albumin or serum.
StabilityThe lyophilized protein isstable for at least 6 months if stored at -80 °C. Reconstituted protein is stable for at least 2weeks at 4 °C, but should be stored in aliquots at -80 °C forlonger term. Avoidrepeated freeze/thaw.
PurityGreater than 85% as determined by SDS-PAGE and HPLC analysis
Biological ActivityThe activity was determined by using a TCF reporter gene assay in 293 cells transfected with Frizzled-4 and LRP-5.WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay.
Country of OriginUSA

WNT-5a belongs to the class of WNT proteins thatactivate the “non-canonical” pathway. Thepredicted size of human WNT-5a is a monomeric protein containing 357 amino acidresidues. Due to glycosylation, itmigrates at an apparent molecular weight of ~45 kDa on SDS-PAGE undernon-reducing conditions. StemRD’sproduct is expressed from a human cell line in animal-free medium, and purifiedwith a proprietary process that is distinct from the published method.

Product Sheet
Yamada A, Iwata T, Yamato M et al., Diverse functionsof secreted frizzled-related proteins in the osteoblastogenesis of human multipotentmesenchymal stromalcells. Biomaterials. 2013 Apr;34(13):3270-8.http://www.ncbi.nlm.nih.gov/pubmed/23384792
品牌介绍
stemRD及其合作伙伴已经开发出一种高效的方法,可以在人细胞系中表达生物活性重组蛋白。由于这些细胞在无血清,无蛋白质和化学成分明确的培养基中生长,因此通过此过程制得的产品不含异种,并且具有人类特异性的转录后修饰,例如糖基化。使用该系统,我们已经能够生产出许多对干细胞的生长和分化至关重要的生物活性蛋白。