Stemrd/WNT-3a，小鼠/5µg/W3A-M-005
市场价：
¥3900.00
美元价：
1950.00
产品分类：
抗仓鼠
公司分类：
Anti_Armenian_Hamster
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Source | M.W. | ~40 kDa | CAS No. | ||
|---|---|---|---|---|---|
| Structural Info | |||||
| Formulation | Lyophilized in sterile filtered solution of PBS with 1% CHAPS | ||||
| Reconstitution | Before reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containing carrierproteins, such as albumin or serum. | ||||
| Stability | The lyophilized protein isstable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least 1month at 4 °C, but should be stored in aliquots at -80°C for longerterm. Avoid repeated freeze andthaw. | ||||
| Purity | Greater than 90% as determined by SDS-PAGE analysis | ||||
| Biological Activity | The activity was determined by using a TCF reporter gene assay in NIH3T3 cells.The EC50 ranges from 50 - 150 ng/ml. | ||||
| Country of Origin | USA | ||||
WNT-3a is a member of the WNT family ofsignaling proteins that play a key role in embryonic development and theintegrity of adult tissues. WNT-3a is aprototypic canonical WNT that signals through the b-catenin pathway. The predicted size of mouse WNT-3a is amonomeric protein containing 333 amino acid residues. Due to glycosylation, it migrates at anapparent molecular weight of 40 kDa by SDS-PAGE analysis under non-reducingconditions. StemRD’s product isexpressed from a mouse cell line overexpressing mouse WNT-3a. Purification is performed with a proprietaryprocess that is distinct from the published method.
Product Sheet
Mae S, Shono A, Shiota F etal., Monitoring and robust induction of nephrogenic intermediatemesoderm from human pluripotent stem cells. Nature Communication. 2013;4:1367. http://www.ncbi.nlm.nih.gov/pubmed/23340407Chu, M. L. H., Ahn, V. E., Choi, H. J., et al., Structural Studies of Wnts andIdentification of an LRP6 Binding Site. Structure., 2013http://www.ncbi.nlm.nih.gov/pubmed/23791946
品牌介绍
stemRD及其合作伙伴已经开发出一种高效的方法，可以在人细胞系中表达生物活性重组蛋白。由于这些细胞在无血清，无蛋白质和化学成分明确的培养基中生长，因此通过此过程制得的产品不含异种，并且具有人类特异性的转录后修饰，例如糖基化。使用该系统，我们已经能够生产出许多对干细胞的生长和分化至关重要的生物活性蛋白。
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