Stemrd/WNT-3a, human/25 µg/W3A-H-025
市场价：
¥21900.00
美元价：
10950.00
产品分类：
小分子
公司分类：
Small_molecule
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Source | M.W. | 38 - 41 kDa | CAS No. | ||
|---|---|---|---|---|---|
| Structural Info | |||||
| Formulation | Lyophilized in sterile filtered solution of PBS with 1% CHAPS | ||||
| Reconstitution | Before reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containingcarrier proteins, such as albumin or serum. | ||||
| Stability | The lyophilized protein isstable for at least 6 months if stored at -80 °C. Reconstituted protein is stable for at leasttwo weeks at 4 °C, but should be stored in aliquots at -80 °C forlonger term. Avoidrepeated freeze and thaw. | ||||
| Purity | Greater than 90% as determined by SDS-PAGE and HPLC analysis | ||||
| Biological Activity | The activity was determined by using a TCF reporter gene assay in cultured human cells.The EC50 ranges from 50 - 150 ng/ml. | ||||
| Country of Origin | USA | ||||
WNT-3a is a member of the WNT family ofsignaling proteins that play key roles in embryonic development and the maintenanceof adult tissues. WNT-3a is a prototypiccanonical WNT that signals through the b-catenin pathway. The predicted size of human WNT-3a is amonomeric protein containing 328 amino acid residues. Due to glycosylation, it migrates at anapparent molecular weight of 38 - 41 kDa on SDS-PAGE under non-reducingconditions. StemRD’s WNT-3a is producedfrom a human cell line overexpressing human Wnt-3a cDNA in protein-free medium. Purification is done by a proprietary processthat is distinct from the published method.
Product Sheet
Kim SE, Huang H, Zhao M, et al., WntStabilization of β-Catenin Reveals Principles for Morphogen Receptor-ScaffoldAssemblies. Science. 2013 May 17; 340(6134):867-70http://www.ncbi.nlm.nih.gov/pubmed/23579495Zhang X, Abreu JG, Yokota C, et al., Tiki1 isrequired for head formation via Wnt cleavage-oxidation and inactivation. Cell. 2012 Jun 22;149(7):1565-77. http://www.ncbi.nlm.nih.gov/pubmed/22726442Hoshiba T, Kawazoe N, Chen G. Mechanism ofregulation of PPARG expressionof mesenchymal stem cells by osteogenesis-mimickingextracellular matrices. Biosci Biotechnol Biochem.2011;75(11):2099-104.http://www.ncbi.nlm.nih.gov/pubmed/22056426
品牌介绍
stemRD及其合作伙伴已经开发出一种高效的方法，可以在人细胞系中表达生物活性重组蛋白。由于这些细胞在无血清，无蛋白质和化学成分明确的培养基中生长，因此通过此过程制得的产品不含异种，并且具有人类特异性的转录后修饰，例如糖基化。使用该系统，我们已经能够生产出许多对干细胞的生长和分化至关重要的生物活性蛋白。
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