Stemrd/TGF-β1, human recombinant/25 µg/TGFB1-025
市场价：
¥14980.00
美元价：
7490.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Source | M.W. | 25 kDa | CAS No. | ||
|---|---|---|---|---|---|
| Structural Info | |||||
| Formulation | Lyophilized in sterile filtered solution of acetonitrile/TFA and PBS | ||||
| Reconstitution | Before reconstitution, we recommend abrief spin to drive down any material dislodged from the bottom of thetube. The lyophilized protein shouldbe reconstituted in 5 mM HCl. Furtherdilutions should be made in buffer or medium containing carrier proteins,such as albumin or serum. | ||||
| Stability | The lyophilized protein isstable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least 1month at 4°C, but should be stored in aliquots at -80 °C forlonger term. Avoidrepeated freeze and thaw. | ||||
| Purity | Greater than 95% as determined by SDS-PAGE and HPLC analyses | ||||
| Biological Activity | The activity was determined by using a SMAD reporter gene assay in human 293 cells. The EC50 ranges from 0.1 – 1.0 ng/ml. | ||||
| Country of Origin | USA | ||||
One of the three closely-related members of the TGF-β family. Human TGF-β1 cDNA encodes a 390 aa precursor with a 29 aa signal peptide. A furin-like convertase cleaves the precursor to form a 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-β1. Disulfide-linked mature TGF-beta1 is dissociated from the LAP upon activation.
StemRD, produces active TGF-β1 in human 293 cells in Xeno-free medium. After a series of chromatography, the protein is purified to near homogeneity. Activity is determined by using a SMAD reporter gene assay with EC50 0.1 – 1.0 ng/mL.
HPLCand SDS-PAGE profiles of TGF-β1, human from StemRD Inctgfb, tgf b, tgf beta
Product Sheet
Eyckmans J, Lin GL, Chen CS. Adhesive and mechanicalregulation of mesenchymal stem Cell differentiationin human bone marrow andperiosteum-derived progenitor cells. Biol Open.2012 Nov 15;1(11):1058-68.http://www.ncbi.nlm.nih.gov/pubmed/23213385
品牌介绍
stemRD及其合作伙伴已经开发出一种高效的方法，可以在人细胞系中表达生物活性重组蛋白。由于这些细胞在无血清，无蛋白质和化学成分明确的培养基中生长，因此通过此过程制得的产品不含异种，并且具有人类特异性的转录后修饰，例如糖基化。使用该系统，我们已经能够生产出许多对干细胞的生长和分化至关重要的生物活性蛋白。
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