Stemrd/声波刺猬，人/25µg/SHH-025
市场价：
¥19900.00
美元价：
9950.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Source | Human Mammalian Cells | M.W. | ~45kDa | CAS No. | |
|---|---|---|---|---|---|
| Structural Info | |||||
| Formulation | Lyophilized in sterile filtered solution of PBS with 1% CHAPS | ||||
| Reconstitution | Before reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containingcarrier proteins, such as albumin or serum. | ||||
| Stability | The lyophilized protein isstable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least 1 month at 4 °C, but shouldbe stored in aliquots at -80 °C for longer term. Avoid repeated freeze andthaw. | ||||
| Purity | Greater than 85% as determined by SDS-PAGE and HPLC analysis | ||||
| Biological Activity | The activity was determined by using a Gli reporter gene assay in 3T3 cells.The EC50 ranges from 5 to 20 ng/ml. | ||||
| Country of Origin | USA | ||||
The hedgehog (Hh) gene encodes a secretedprotein originally identified in Drosophila. The vertebrate homologs of Hhcomprise of Sonic (SHh), Desert (DHh) and Indian (IHh). SHh plays an important role in embryonicdevelopment and in diseases such as cancer. Human SHh precursor is a45kDa protein. An autocatalytic reaction yields a 19 kDa N-terminaldomain that is biologically active. Because of unique post-translational modifications, SHh produced inmammalian cells is much more biologically active than SHh produced from E. coliand is devoid of artifact associated with the E. coli products commerciallyavailable from other manufacturers.
 | SHh from StemRD made in human cells is 10-50 times more active, than other commercial products made in E. coli.Activity is detected by a Gli reporter gene assay in Swiss 3T3 cells. |
Product Sheet
Chen Y, Yue S, Xie L, et al., DualPhosphorylation of suppressor of fused (Sufu) by PKA and GSK3beta regulates itsstability and localizationin the primary cilium. J Biol Chem. 2011 Apr 15;286(15):13502-11.http://www.ncbi.nlm.nih.gov/pubmed/21317289Ding H, Zhou D, Hao S, etal., Sonic hedgehog signaling mediates epithelial-mesenchymalcommunication and promotes renal fibrosis. JAm Soc Nephrol. 2012 May;23(5):801-13.http://www.ncbi.nlm.nih.gov/pubmed/22302193Wang J, Mook RA Jr, Lu J, et al., Identification of a novel Smoothenedantagonist that potently suppresses Hedgehog signaling. Bioorg Med Chem. 2012 Nov 15;20(22):6751-7. http://www.ncbi.nlm.nih.gov/pubmed/23063522Okabe S, Tauchi T, TanakaY, et al., Effects of the hedgehog inhibitor GDC-0449, alone or in combination with dasatinib, onBCR-ABL-positive leukemia cells. Stem Cells Dev. 2012 Nov1;21(16):2939-48.http://www.ncbi.nlm.nih.gov/pubmed/22642671
品牌介绍
stemRD及其合作伙伴已经开发出一种高效的方法，可以在人细胞系中表达生物活性重组蛋白。由于这些细胞在无血清，无蛋白质和化学成分明确的培养基中生长，因此通过此过程制得的产品不含异种，并且具有人类特异性的转录后修饰，例如糖基化。使用该系统，我们已经能够生产出许多对干细胞的生长和分化至关重要的生物活性蛋白。
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