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公司介绍
stemRD及其合作伙伴已经开发出一种高效的方法,可以在人细胞系中表达生物活性重组蛋白。由于这些细胞在无血清,无蛋白质和化学成分明确的培养基中生长,因此通过此过程制得的产品不含异种,并且具有人类特异性的转录后修饰,例如糖基化。使用该系统,我们已经能够生产出许多对干细胞的生长和分化至关重要的生物活性蛋白。
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  • URL:http://stemrd.com/

Stemrd/R-spondin-1,重组人/25µg/RSPO-025

  • 产品编号: RSPO-025
  • 美  元  价: 2910.00
  • 市  场  价: ¥5820.00
  • 品       牌: Stemrd
  • 产       地: 美国(厂家直采)
  • 公       司:
  • 产品分类: 常用设备>
  • 公司分类: Common_equipment
Stemrd/R-spondin-1,重组人/25µg/RSPO-025
  • Stemrd/R-spondin-1,重组人/25µg/RSPO-025
商品介绍
Sourcehuman 293 cells M.W.25.6 kDaCAS No.
Structural Info
FormulationLyophilized in sterile filtered solution of PBS.
ReconstitutionBefore reconstitution, a brief spin is recommend todrive down any material dislodged from the bottom of the tube. The lyophilized protein should be reconstitutedin sterile H2O to a desired concentration.
StabilityThe lyophilized protein is stable for at least oneyear if stored at -80 °C. Reconstituted protein is stable for at least four weeks at 4 °C,but should be stored in aliquots at -80 °C for longer term. Avoidrepeated freeze and thaw.
PurityGreater than 90% as determined by SDS-PAGE analysis
Biological ActivityThe activity was determined by using a TCF reporter gene assay in cultured human cells.The EC50 ranges from 5 - 20 ng/ml in the presence of 10 ng/mL human WNT-3a.
Country of OriginUSA

R-spondin-1 (RSPO-1)is a natural enhancer of the canonicalWNT pathway. When used together with WNT proteins that activate thebeta-catenin pathway, R-spondin-1 enhances the activity of canonical WNTproteins by binding to LGR5 and LGR4 (refs.)Injection of recombinant R­Spondin-1 intomouse causes activation of the β­catenin pathway and proliferation of intestinal crypt cells, which forms the basis for aclinical trial in amelioration of chemotherapy-induced colitis.ThePredicted MW of full-length R-Spondin-1 is 25.6 kDa, anddue to glycosylation it runs on SDS­PAGE at ~39 kDa underreducing conditions. StemRD’sR-Spondin-1 is produced in human 293 cells as a secreted protein (without tag)and purified by a series of chromatography.Refs: de Lau, et al, LGR5 homologuesassociate with Wnt receptors and mediate R-spondin signaling. Nature. 2011 July 4; 476: 293; Carmon, et al, R-spondins function as ligandsfor the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling.PNAS. 2011 Jul 12; 108: 11452.

Product Sheet
Kawamura M, Miyagawa S, Miki K et al., Feasibility, safety, and therapeutic efficacy of human induced pluripotent stemcell-derived cardiomyocyte sheetsin a porcine ischemiccardiomyopathy model. Circulation. 2012 Sep 11;126(11 Suppl 1):S29-37.http://www.ncbi.nlm.nih.gov/pubmed/22965990
品牌介绍

stemRD及其合作伙伴已经开发出一种高效的方法,可以在人细胞系中表达生物活性重组蛋白。由于这些细胞在无血清,无蛋白质和化学成分明确的培养基中生长,因此通过此过程制得的产品不含异种,并且具有人类特异性的转录后修饰,例如糖基化。使用该系统,我们已经能够生产出许多对干细胞的生长和分化至关重要的生物活性蛋白。

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