Stemrd/Noggin，重组人/1000µg/NOGG-1000
市场价：
¥59900.00
美元价：
29950.00
产品分类：
制冰机
公司分类：
ice_machine
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Source | Human 293 cells | M.W. | 24.6 kDa | CAS No. | |
|---|---|---|---|---|---|
| Structural Info | |||||
| Formulation | Lyophilized in sterile filtered solution of PBS, pH7.4, 10% trehalose (stabilizer) | ||||
| Reconstitution | Before reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O. Further dilutions should be made in buffer or medium containing carrierproteins, such as albumin or serum. | ||||
| Stability | The lyophilized protein isstable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least 1 month at 4 °C, but shouldbe stored in aliquots at -80 °C for longer term. Avoid repeated freeze/thaw. | ||||
| Purity | Greater than 95% by SDS-PAGE | ||||
| Biological Activity | The activity was determined by using a reporter gene assay in human 293 cells for the inhibition of activity of BMP4 (2.5 ng/mL).The EC50 ranges from 25 - 100 ng/mL. | ||||
| Country of Origin | USA | ||||
Noggin has been shown to be ahigh-affinity binding protein to BMP and antagonizes BMP bio-activities. It can be added to cell culture medium forembryonic stem cells to maintain pluripotency.The recombinant human Noggin comprises 216 aminoacids after removal of the signal peptide and has a predicted molecular mass of24.6 kDa. As a result of glycosylation, itmigrates as an approximately 30 kDa band in SDS-PAGE under reducing conditionsStemRD"s Noggin ismade in human 293 cells, which renders human-specific post-translationalmodifications. We purified this product through a series ofchromatography.
Product Sheet
Liu J, Koscielska KA, Cao Z, et al., Signaling defects in iPSC-derived fragileX premutation neurons. Hum Mol Genet.2012 Sep 1;21(17):3795-805.http://www.ncbi.nlm.nih.gov/pubmed/22641815
品牌介绍
stemRD及其合作伙伴已经开发出一种高效的方法，可以在人细胞系中表达生物活性重组蛋白。由于这些细胞在无血清，无蛋白质和化学成分明确的培养基中生长，因此通过此过程制得的产品不含异种，并且具有人类特异性的转录后修饰，例如糖基化。使用该系统，我们已经能够生产出许多对干细胞的生长和分化至关重要的生物活性蛋白。
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