Stemrd/Frizzled-5 Fc融合，人/250µg/HFZ5FC-250
商品编号：
HFZ5FC-250
品牌：
Stemrd
市场价：
¥18100.00
美元价：
9050.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Source | CHO cells | M.W. | CAS No. | ||
|---|---|---|---|---|---|
| Structural Info | |||||
| Formulation | Lyophilized in sterile filtered solution of PBS. | ||||
| Reconstitution | Before reconstitution, abrief spin is recommend to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a desired concentration. | ||||
| Stability | The lyophilized protein isstable for at least one year if stored at -80 °C. Reconstituted protein is stable forat least four weeks at 4 °C, but should be stored in aliquots at -80°C for longer term. Avoidrepeated freeze and thaw. | ||||
| Purity | Greater than 95% by SDS-PAGE analysis | ||||
| Biological Activity | The activity was determined by using a TCF reporter gene assay in cultured human cells.The IC50 ranges from 100 - 200 ng/ml in the inhibition of 100 ng/mL WNT-3a activity. | ||||
| Country of Origin | USA | ||||
Frizzled-5 is one of the 10 mammalian Frizzled receptors for WNTproteins. The extracellular cycteine rich domain of Frizzleds bind toWNTs with nM affinity. When supplied as recombinant protein, thisextracellular domain of Frizzled-5 acts as a soluble receptor, blocking thebinding of WNTs to Frizzled on the cells.
StemRD’s Frizzled-5 extracellular domain protein is fused to theN-terminus of the Fc domain of human IgG1. This fusion allows stabilizationof the protein in vitro and in vivo and dimerization of the recombinant proteinrendering high-affinity binding to WNTs. A similar protein, Frizzled-8 Fc fusion, has been used byGenentech scientists to show anti-tumor effect of WNT blockade in animal models(DeAlmeida VI et al, Cancer Res. 2007, 67:5371).
Expression is from CHO cells cultured in serum-free and protein-freemedium. Purification of the fusionprotein from conditioned culture medium is performed using protein Achromatography.
Frizzled-5 Fc fusion, human recombinant
品牌介绍
stemRD及其合作伙伴已经开发出一种高效的方法，可以在人细胞系中表达生物活性重组蛋白。由于这些细胞在无血清，无蛋白质和化学成分明确的培养基中生长，因此通过此过程制得的产品不含异种，并且具有人类特异性的转录后修饰，例如糖基化。使用该系统，我们已经能够生产出许多对干细胞的生长和分化至关重要的生物活性蛋白。
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