Cygnus-_Technologies F650 HEK 293 细胞 HCP ELISA 试剂盒_ELISA...

来自 : 发布时间：2024-04-29

产品英文名称：HEK 293 HCP ELISA kit产品中文名称：HEK 293 细胞 ELISA 试剂盒HEK 293 HCP ELISA kit 的应用本试剂盒是用于确定在HEK 293细胞表达产品中宿主细胞蛋白污染的存在。该试剂盒是用于研究和制造使用，不用于人类或动物的诊断应用。这是HEK HCP检测第二代ELISA试剂盒。这种新的试剂盒，取代原来的F150 ELISA试剂盒，不再是市售。第二代测定使用一个更广泛的反应性抗体。这样的试剂盒可以产生与使用旧F150试剂盒不同的HEK HCP污染水平的报告。HEK 293 细胞 ELISA 试剂盒的英文描述Cygnus is pleased to announce the re-introduction of HEK 293 Host Cell Protein ELISA kit. This 2nd Generation kit utilizes antibodies derived from the same pool of goat anti-HEK antiserum used in the 1st Generation kit, however this antiserum has now been affinity purified with new and a more represenatitve preparation of HCPs typically found in virus, vaccine and recombinant protein products.HEK 293 HCP ELISA kit 的使用步骤规则The HEK assay is a two-site immunoenzymetric assay. Samples containing HEK HCPs are reacted simultaneously with a horseradish peroxidase (HRP) enzyme labeled anti-HEK antibody (goat polyclonal) in microtiter strips coated with an affinity purified capture anti-HEK antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of HEK HCPs present.HEK 293 HCP ELISA kit 的保存和稳定性* All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.* The substrate reagent should not be used if its stopped absorbance at 450nm is greater than 0.1.* Reconstituted wash solution is stable until the expiration date of the kit.HEK 293 HCP ELISA kit 的使用注意事项*只用于研究或制造。*停止反应试剂是0.5N H2SO4。避免接触眼睛，皮肤，和服装。在用本试剂盒的试剂浓度，其他没有被认为是有害的。*本试剂盒只能由合格的技术人员使用。一切干细胞实验耗材优惠尽在华雅思创生物2015年统统促销价，--一切活动尽在上海华雅思创！：，客服StemRDMGro-500MSCmedium,chemically-defined,间充质干细胞培养基500mL/瓶LONZA04-418QX-VIVO15无血清培养基1L/瓶LifeTechnology087-0112DKAIM-V?CTS?无血清细胞培养基（细胞治疗级）1000mL/瓶Pall15950-017UltroserSerumSubstitute血清替代品20mL/瓶LONZA12-725FUltraCULTURESerum-freeMedium无血清培养基500mL/瓶BD 762165 PAXgeneBloodRNATubeRNA样本收集管 100管/盒LONZA 50150 SeaKem?GoldAgarose金琼脂糖粉 125g/瓶LONZA CC-3162 EGM-2BulletKit(CC-3156 CC-4176)套装 Kit/套Nunc 159910 175cm2易用培养瓶,PS,已灭菌,瓶盖:过滤盖 30个/箱LONZA LT07-318MycoAlert?MycoplasmaDetectionKit支原体检测试剂盒 100Tests/盒LONZA LT07-418 MycoAlert?MycoplasmaDetectionKit支原体检测试剂盒 50Tests/盒Lonza 50-650U Kinetic-QCL192TestKit内毒素检测试剂盒 192T/盒Corning431080175cm⒉细胞培养瓶，Angled颈，透气盖50个/箱Corning8388125mL储液瓶StorageBottles,PS,45mm瓶颈，带盖，灭菌，独立包装24个/箱Corning4306592ml冻存管,聚丙烯，外旋盖,可自立圆底500个/箱Corning329075cm2CellBINDSurface培养瓶，已灭菌，透气盖100个/箱Corning3292175cm2CellBINDSurface培养瓶，已灭菌，透气盖50个/箱BD-Falcon352360CellStrainer,100μm,Yellow100um细胞滤网50个/箱BDFalcon 352070 50mL锥形离心管，高透明度聚丙烯,袋装 500支/箱BDFalcon 352096 15mL锥形离心管，高透明度聚丙烯,袋装 500支/箱BDBioCoat 354234 MatrigelBasementMembraneMatrix(标准型) 10mL/瓶Cygnus F550 CHOHCPELISAKit,3G宿主蛋白检测试剂盒 96T/盒Cygnus F410 E.coliHCPELISAKit大肠杆菌 96T/盒Nalgene 5100-0001 Cryo1℃FreezingContainer梯度降温冻存盒 1个/箱Nalgene5026-0909CryoBoxes冻存盒9X9阵列，管瓶1.2/2.0mL24个/箱Nalgene2250-0130 50L可高温高压操作的细口大瓶（带手柄） 1个/箱Nalgene 2319-005020L可高温高压灭菌的细口大瓶（带放水口）1个/箱Hyclone SH30084.03 FBS优等澳洲胎牛血清 500mL/瓶PeproTech100-18B-500 RecombinantHumanFGF-basic(154a.a.)因子 500ug/支Parafilm PM-996 Parafilm?MLaboratoryFilm封口膜 12卷/箱